Fluorescent Probes and Selective Inhibitors for Biological Studies of Biology Diagrams

Fluorescent Probes and Selective Inhibitors for Biological Studies of Biology Diagrams The expression levels of two closely linked proteins in the eukaryotic DNA replication network came in handy for Miyawaki's team when visualizing the cell cycle. Chromatin licensing and DNA replication factor 1 (Cdt1) "licenses" DNA to replicate during the cell cycle. Geminin inhibits Cdt1, preventing it from re-replicating DNA.

Abstract. Proper cell-cycle progression is essential for the self-renewal and differentiation of human pluripotent stem cells (hPSCs). The fluorescent ubiquitination-based cell-cycle indicator (FUCCI) has allowed the dual-color visualization of the G 1 and S/G 2 /M phases in various dynamic models, but its application in hPSCs is not widely reported. In addition, lineage-specific FUCCI

PDF Real Time Visualization of the Cell Biology Diagrams

Fucci (Fluorescent Ubiquitination-based Cell Cycle Indicator)is a set of fluorescent probes which enable the visualization of cell cycle progression in living cells. Fucci takes advantage of the fact that the replication licensing factors Cdt1 and Geminin are only present during specific phases of the cell cycle. A fusion protein of a fragment

The cell-cycle transition from G1 to S phase has been difficult to visualize. We have harnessed antiphase oscillating proteins that mark cell-cycle transitions in order to develop genetically encoded fluorescent probes for this purpose. These probes effectively label individual G1 phase nuclei red and those in S/G2/M phases green. We were able to generate cultured cells and transgenic mice The widely used fluorescence ubiquitination cell cycle indicator (FUCCI) system, based on the expression of the cell cycle oscillators Cdt1 and geminin tagged with different fluorescent proteins By visualizing the cell cycle, Fucci is a powerful tool to investigate any process that has to do with cell growth and differentiation, such as the development and regeneration of organs as well as carcinogenesis. Cells with pure green fluorescence were either in the S or G2 phase and were distinguished by immunostaining of the S phase. The

Fluorescent indicators for continuous and lineage Biology Diagrams

A robust method for simultaneous visualization of all four cell cycle phases in living cells is highly desirable. We developed an intensiometric reporter of the transition from S to G2 phase and