Molecular Expressions Cell Biology Mitosis with Fluorescence Microscopy Biology Diagrams

Molecular Expressions Cell Biology Mitosis with Fluorescence Microscopy Biology Diagrams The expression levels of two closely linked proteins in the eukaryotic DNA replication network came in handy for Miyawaki's team when visualizing the cell cycle. Chromatin licensing and DNA replication factor 1 (Cdt1) "licenses" DNA to replicate during the cell cycle. Geminin inhibits Cdt1, preventing it from re-replicating DNA.

Fucci (CA) yields abundant expression of mCherry throughout G1 phase with an abrupt decrease in the red fluorescence at the end of G1 phase. Fucci (CA) can be used to reliably detect a short G1 phase and distinguish S and G2 phases, which previously was difficult to achieve. (CA) Figure1: Visualization of Cell-Cycle Progression by Fucci (SA

Visualizing Spatiotemporal Dynamics of Multicellular Cell Biology Diagrams

A robust method for simultaneous visualization of all four cell cycle phases in living cells is highly desirable. We developed an intensiometric reporter of the transition from S to G2 phase and

Fucci (Fluorescent Ubiquitination-based Cell Cycle Indicator)is a set of fluorescent probes which enable the visualization of cell cycle progression in living cells. Fucci takes advantage of the fact that the replication licensing factors Cdt1 and Geminin are only present during specific phases of the cell cycle. A fusion protein of a fragment

Development of a New Fucci(CA) Application: A Fluorescent Probe for ... Biology Diagrams

By visualizing the cell cycle, Fucci is a powerful tool to investigate any process that has to do with cell growth and differentiation, such as the development and regeneration of organs as well as carcinogenesis. Cells with pure green fluorescence were either in the S or G2 phase and were distinguished by immunostaining of the S phase. The